Type

Recombinant

Description

Matrixmetalloproteinases(MMPs)areafamilyofendoproteasesthatrequirezincandcalciumforexpressingcatalyticactivity.Theseenzymesplayacentralroleinthemaintenanceandremodelingoftheextracellularmatrix.Elevatedexpressionoftheiractivity,causedeitherbyup-regulationoftheirexpressionordown-regulationoftheircognateinhibitors,hasbeenimplicatedinvariousdegenerativedisorders,includingarthritis,cardiovasculardisease,skeletalgrowth-platedisorders,andcancermetastasis.MMP-3 degradesfibronectin,laminin,collagensIII,IV,andX,andcartilageproteoglycans.RecombinantHumanMMP-3 isa42.8 kDaproteincontainingtheentirecatalyticN-terminaldomainandtheC-terminaldomain(378 aminoacids).

AminoAcidSequence

MRTFPGIPKWRKTHLTYRIVNYTPDLPKDAVDSAVEKALKVWEEVTPLTFSRLYEGEADIMISFAVREHGDFYPFDGPGNVLAHAYAPGPGINGDAHFDDDEQWTKDTTGTNLFLVAAHEIGHSLGLFHSANTEALMYPLYHSLTDLTRFRLSQDDINGIQSLYGPPPDSPETPLVPTEPVPPEPGTPANCDPALSFDAVSTLRGEILIFKDRHFWRKSLRKLEPELHLISSFWPSLPSGVDAAYEVTSKDLVFIFKGNQFWAIRGNEVRAGYPRGIHTLGFPPTVRKIDAAISDKEKNKTYFFVEDKYWRFDEKRNSMEPGFPKQIAEDFPGIDSKIDAVFEEFGFFYFFTGSSQLEFDPNAKKVTHTLKSNSWLNC

Source

E.coli

Purity

98%

BIOLOGicalActivity

MMP-3 activitywasmeasuredbyitsABIlitytocleaveachromogenicpeptideMMP-3 substrateatroomtemperature.AtaMMP-3 concentrationof2.5 µg/ml,50%cleavagewasachievedatanincubationtimeofapproximately75 minutes.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.5–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C