Type

Recombinant

Description

Matrixmetalloproteinases(MMPs)areafamilyofendoproteasesthatrequirezincandcalciumforexpressingcatalyticactivity.Theseenzymesplayacentralroleinthemaintenanceandremodelingoftheextracellularmatrix.Elevatedexpressionoftheiractivity,causedeitherbyup-regulationoftheirexpressionordown-regulationoftheircognateinhibitors,hasbeenimplicatedinvariousdegenerativedisorders,includingarthritis,cardiovasculardisease,skeletalgrowth-platedisorders,andcancermetastasis.MMP-2 isasecretedCollagenasewithspecificitytowardTypeIV,V,VII,andXcollagens.RecombinantHumanMMP-2 isa62.0 kDaproteincontainingtheentirecatalyticN-terminaldomainandtheC-terminaldomain(552 aminoacids).

AminoAcidSequence

MYNFFPRKPKWDKNQITYRIIGYTPDLDPETVDDAFARAFQVWSDVTPLRFSRIHDGEADIMINFGRWEHGDGYPFDGKDGLLAHAFAPGTGVGGDSHFDDDELWTLGEGQVVRVKYGNADGEYCKFPFLFNGKEYNSCTDTGRSDGFLWCSTTYNFEKDGKYGFCPHEALFTMGGNAEGQPCKFPFRFQGTSYDSCTTEGRTDGYRWCGTTEDYDRDKKYGFCPETAMSTVGGNSEGAPCVFPFTFLGNKYESCTSAGRSDGKMWCATTANYDDDRKWGFCPDQGYSLFLVAAHEFGHAMGLEHSQDPGALMAPIYTYTKNFRLSQDDIKGIQELYGASPDIDLGTGPTPTLGPVTPEICKQDIVFDGIAQIRGEIFFFKDRFIWRTVTPRDKPMGPLLVATFWPELPEKIDAVYEAPQEEKAVFFAGNEYWIYSASTLERGYPKPLTSLGLPPDVQRVDAAFNWSKNKKTYIFAGDKFWRYNEVKKKMDPGFPKLIADAWNAIPDNLDAVVDLQGGGHSYFFKGAYYLKLENQSLKSVKFGSIKSDWLGC

Source

E.coli

Purity

98%

BIOLOGicalActivity

MMP-2 activitywasmeasuredbyitsABIlitytocleaveachromogenicpeptideMMP-2 substrateatroomtemperature.AtanMMP-2 concentrationof2.5 µg/ml,50%cleavagewasachievedatanincubationtimeofapproximately25 minutes.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.5–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C