Type

Recombinant

Description

Matrixmetalloproteinases(MMPs)areafamilyofendoproteasesthatrequirezincandcalciumforexpressingcatalyticactivity.Theseenzymesplayacentralroleinthemaintenanceandremodelingoftheextracellularmatrix.Elevatedexpressionoftheiractivity,causedeitherbyup-regulationoftheirexpressionordown-regulationoftheircognateinhibitors,hasbeenimplicatedinvariousdegenerativedisorders,includingarthritis,cardiovasculardisease,skeletalgrowth-platedisorders,andcancermetastasis.MMP-1 isasecretedcollagenasewithspecificitytowardTypeI,II,III,VII,andXcollagens.RecombinantHumanMMP-1 isa42.7 kDaproteincontainingtheentirecatalyticN-terminaldomainandtheC-terminaldomain,whichisinvolvedinsubstratespecificity,andinbindingTIMP-1.

AminoAcidSequence

MFVLTEGNPRWEQTHLTYRIENYTPDLPRADVDHAIEKAFQLWSNVTPLTFTKVSEGQADIMISFVRGDHRDNSPFDGPGGNLAHAFQPGPGIGGDAHFDEDERWTNNFREYNLHRVAAHELGHSLGLSHSTDIGALMYPSYTFSGDVQLAQDDIDGIQAIYGRSQNPVQPIGPQTPKACDSKLTFDAITTIRGEVMFFKDRFYMRTNPFYPEVELNFISVFWPQLPNGLEAAYEFADRDEVRFFKGNKYWAVQGQNVLHGYPKDIYSSFGFPRTVKHIDAALSEENTGKTYFFVANKYWRYDEYKRSMDPGYPKMIAHDFPGIGHKVDAVFMKDGFFYFFHGTRQYKFDPKTKRILTLQKANSWFNCRKN

Source

E.coli

Purity

90%

BIOLOGicalActivity

MMP-1 activitywasmeasuredbyitsABIlitytocleaveachromogenicpeptideMMP-1 substrateatroomtemperature.AtanMMP-1 concentrationof2.5 µg/ml,50%cleavagewasachievedatanincubationtimeofapproximately25 minutes.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.5–1.0 mg/ml.Note:Slowtodissolve.Gentlyshakethesolutionforabout10 minutesforbettersolubility.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C