Type

Recombinant

Description

MIP-5 isaCCchemokinethatisexpressedintheheart,skeletalmuscleandadrenalgland.MIP-5 primarilysignalsthroughtheCCR1receptor,buthasalsobeenfoundtobindtoCCR3.MIP-5 ischemotactictowardsTcellsandmonocytes.RecombinantHumanMIP-5 isa10.1 kDaproteincontaining92 aminoacidresidues,includingthefourhighlyconservedcysteineresiduespresentinCCchemokines.

AminoAcidSequence

QFTNDAETELMMSKLPLENPVVLNSFHFAADCCTSYISQSIPCSLMKSYFETSSECSKPGVIFLTKKGRQVCAKPSGPGVQDCMKKLKPYSI

Source

E.coli

Purity

98%

BIOLOGicalActivity

DeterminedbyitsABIlitytochemoattracthumanTlymphocytesusingaconcentrationrangeof1.0–10.0 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C