Type

Recombinant

Description

MIP-4 isaCCchemokinethatisexpressedinlymphnodes,lungs,placentaandbonemarrow.MIP-4"s primaryreceptorisunknown.MIP-4 chemoattractslymphocytes,andhasbeenshowntoexertactivityonbothCD4+andCD8+Tcells.RecombinantHumanMIP-4 isa7.8 kDaproteincontaining68 aminoacidresidues,includingthefourhighlyconservedcysteineresiduespresentinCCchemokines.

AminoAcidSequence

AQVGTNKELCCLVYTSWQIPQKFLVDYSETSPQCPKPGVILLTKRGRQICADPNKKWVQKYISDLKLNA

Source

E.coli

Purity

98%

BIOLOGicalActivity

Assay#1:DeterminedbyitsABIlitytochemoattracthumanTlymphocytesusingaconcentrationof1.0–10.0 ng/ml.Assay#2:DeterminedbyitsabilitytochemoattracthumaniDCsusingaconcentrationof1.0–10.0 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C