Type

Recombinant

Description

MIP-3βisaCCchemokine,expressedinthethymus,lymphnodes,andinactivatedbonemarrowstromalcellsthatsignalsthroughtheCCR7receptor.MIP-3βisachemoattractantforTandBlymphocytes,andmyeloidProgenitorcells.HumanMIP-3βisactiveonmurinecells.RecombinantHumanMIP-3βisan8.8 kDaproteincontaining77 aminoacidresidues,includingthefourhighlyconservedcysteineresiduespresentinCCchemokines.

AminoAcidSequence

GTNDAEDCCLSVTQKPIPGYIVRNFHYLLIKDGCRVPAVVFTTLRGRQLCAPPDQPWVERIIQRLQRTSAKMKRRSS

Source

E.coli

Purity

98%

BIOLOGicalActivity

DeterminedbyitsABIlitytochemoattracthumanTcellsusingaconcentrationof10.0–50.0 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C