Type

Recombinant

Description

MIP-3αisaCCchemokine,expressedintheliver,lymphnodes,appendix,PBLandlungsthatcansignalthroughtheCCR6receptor.MIP-3αischemotactictowardslymphocytesanddendriticcells.Additionally,itpromotestheadhesionofmemoryCD4+Tcells,andinhibitscolonyformationofbonemarrowmyeloidimmatureProgenitors.RecombinantHumanMIP-3αisan8.0 kDaproteincontaining70 aminoacidresidues,includingthefourhighlyconservedcysteineresiduespresentinCCchemokines.

AminoAcidSequence

ASNFDCCLGYTDRILHPKFIVGFTRQLANEGCDINAIIFHTKKKLSVCANPKQTWVKYIVRLLSKKVKNM

Source

E.coli

Purity

98%

BIOLOGicalActivity

DeterminedbyitsABIlitytochemoattracthumanTcellsusingaconcentrationof10.0–50.0 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C