Type

Recombinant

Description

BothMIP-1αandMIP-1βarestructurallyandfunctionallyrelatedCCchemokines.TheyparticipateinhostresponsetoinvADIngbacterial,viral,parasiteandfungalpathogens,byregulatingthetrafficking,andactivationstate,ofselectedsubgroupsofinflammatorycells(.g.macrophages,lymphocytesandNKcells).WhilebothMIP-1αandMIP-1βexertsimilareffectsonmonocytes,theireffectonlymphocytesdiffer;withMIP-1αselectivelyattractingCD8+lymphocytes,andMIP-1βselectivelyattractingCD4+lymphocytes.Additionally,MIP-1αandMIP-1βhavealsobeenshowntobepotentchemoattractantsforBcells,eosinophilsanddendriticcells.BothhumanandmurineMIP-1αandMIP-1βareactiveonhumanandmurinehematopoieticcells.RecombinantMurineMIP-1βisa7.8 kDaproteincontaining69 aminoacidresidues,includingthefourhighlyconservedcysteineresiduespresentinCCchemokines.

AminoAcidSequence

APMGSDPPTSCCFSYTSRQLHRSFVMDYYETSSLCSKPAVVFLTKRGRQICANPSEPWVTEYMSDLELN

Source

E.coli

Purity

95%

BIOLOGicalActivity

DeterminedbyitsABIlitytochemoattracthumanmonocytesusingaconcentrationrangeof20.0–100.0 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C