Type

Recombinant

Description

MIG,aCXCchemokine,isproducedbyIFN-γstimulatedmonocytes,macrophagesandendothelialcells.ItsignalsthroughtheCXCR3receptor.MIGselectivelychemoattractsTh1lymphocytes,andalsoexertsotheractivities,includinginhibitionoftumorgrowth,angiogenesis,andinhibitionofcolonyformationofhematopoieticProgenitors.HumanMIGisactiveonmurinecells.RecombinantMurineMIGisa12.2 kDaproteincontaining105 aminoacidresidues,includingthefourhighlyconservedcysteineresiduespresentinCXCchemokines.

AminoAcidSequence

TLVIRNARCSCISTSRGTIHYKSLKDLKQFAPSPNCNKTEIIATLKNGDQTCLDPDSANVKKLMKEWEKKINQKKKQKRGKKHQKNMKNRKPKTPQSRRRSRKTT

Source

E.coli

Purity

98%

BIOLOGicalActivity

DeterminedbyitsABIlitytochemoacttracthumanlymphocytesusingaconcentrationrangeof0.1–1.0 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C