Type

Recombinant

Description

TheHumanMidkineiscreatedasarecombinantproteinwithNterminalfusionofHisTag.TheHumanMidkineHis-TaggedFusionProtein,producedinE.coli,is14.6 kDaproteincontaining121 aminoacidresiduesofthehumanMidkineand10 additionalaminoacidresidues –HisTag.UniProtKBacc.no.P21741.

AminoAcidSequence

MKHHHHHHHMKKKDKVKKGGPGSECAEWAWGPCTPSSKDCGVGFREGTCGAQTQRIRCRVPCNWKKEFGADCKYKFENWGACDGGTGTKVRQGTLKKARYNAQCQETIRVTKPCTPKTKAKAKAKKGKGKD

Source

E.coli

Purity

>95%

SDS-PAGEGel

12%SDS-PAGEseparationofHumanMidkine
1. M.W.Marker –14,21,31,45,66,97 kDa
2.reducedandheatedsample,5μg/lane
3.non-reducedandnon-heatedsample,5μg/lane

Formulation

Filtered(0,4 μm)andlyophilizedin0.5 mg/mLin0.05 Mphosphatebuffer,0.1 MNaCl,pH 7.2

Reconstitution

AddPBSpH7.2 toprepareaworkingstocksolutionofapproximately0.5 mg/mLandletthelyophilizedpelletdissolvecompletely.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture

Applications

Westernblotting,ELISA

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4°Cfora week.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.

Note

Thisproductisintendedforresearchuseonly.