Type

Recombinant

Description

MECisasecretedCCchemokineexpressedprimarilybyepithelialcellsofthebronchioles,salivarygland,mammaryglandandcolon.MECsignalsthroughtheCCR10receptor,andchemoattractsrestingCD4,CD8T-cellsandeosinophils.MECcontainssixcysteines,includingthefourhighlyconservedcysteineresiduespresentinCCchemokines.RecombinantMurineMECisa12.6 kDaproteincontaining111 aminoacidresidues.

AminoAcidSequence

SEAILPMASSCCTEVSHHVSGRLLERVSSCSIQRADGDCDLAAVILHVKRRRICISPHNRTLKQWMRASEVKKNGRENVCSGKKQPSRKDRKGHTTRKHRTRGTHRHEASR

Source

E.coli

Purity

98%

BIOLOGicalActivity

DeterminedbyitsABIlitytochemoattractmurinelymphocytesusingaconcentrationrangeof1.0–10.0 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C