Type
Recombinant
Description
M-CSFisapotenthematopoieticfactorproducedbyavarietyofcells,includinglymphocytes,monocytes,fibroblasts,endothelialcells,myoblastsandosteoblasts.Itisakeyregulatorofcellularproliferation,differentiation,andsurvivalforbloodmonocytes,tissuemacrophages,andtheirrespectiveProgenitorcells.M-CSFhasbeenshowntoplayimportantrolesinmodulatingdermalthicknessandfertility.M-CSFisclinicallyusedinthetreatmentofinfection,malignanciesandatherosclerosis.Itfacilitateshematopoieticrecoveryafterbonemarrowtransplantation.HumanM-CSFisreactiveinmurinesystems,butthemurinemoleculeexhibitsnoactivityonhumancells.RecombinantHumanM-CSFisa36.8 kDahomodimericproteinconsistingoftwo159 aminoacidpolypeptidesubunits.
AminoAcidSequence
MEEVSEYCSHMIGSGHLQSLQRLIDSQMETSCQITFEFVDQEQLKDPVCYLKKAFLLVQDIMEDTMRFRDNTPNAIAIVQLQELSLRLKSCFTKDYEEHDKACVRTFYETPLQLLEKVKNVFNETKNLLDKDWNIFSKNCNNSFAECSSQGHERQSEGS
Source
E.coli
Purity
98%
BIOLOGicalActivity
TheED50wasdeterminedbythedose-dependentstimulationoftheproliferationofmurineM-NSF-60 cells1 ng/ml,correspondingtoaspecificactivityof1×106units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–0.5 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
