Type

Recombinant

Description

Maspin(mammaryserineproteaseinhibitor)isanon-inhibitoryserpinthatisexpressedpredominantlyinnormalmammaryepithelialcells,butatsignificantlyreducedlevelsorabsentinmostbreastcarcinomas.IthastheABIlitytoblockthegrowth,invasiveness,andmetastaticpotentialofbreastandlungtumors.Thisanti-tumoractivityisachieved,inpart,bythecontributionofmaspintotheinhibitionofangiogenesis,anditsabilitytopreferentiallypromoteapoptosisoftumorcells.RecombinantHumanMaspinisa42.1 kDanon-glycosylatedproteincontaining375 aminoacidresidues.

AminoAcidSequence

MDALQLANSAFAVDLFKQLCEKEPLGNVLFSPICLSTSLSLAQVGAKGDTANEIGQVLHFENVKDIPFGFQTVTSDVNKLSSFYSLKLIKRLYVDKSLNLSTEFISSTKRPYAKELETVDFKDKLEETKGQINNSIKDLTDGHFENILADNSVNDQTKILVVNAAYFVGKWMKKFPESETKECPFRLNKTDTKPVQMMNMEATFCMGNIDSINCKIIELPFQNKHLSMFILLPKDVEDESTGLEKIEKQLNSESLSQWTNPSTMANAKVKLSIPKFKVEKMIDPKACLENLGLKHIFSEDTSDFSGMSETKGVALSNVIHKVCLEITEDGGDSIEVPGARILQHKDELNADHPFIYIIRHNKTRNIIFFGKFCSP

Source

E.coli

Purity

97%

BIOLOGicalActivity

DatanotAvailable.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat-20°Cto –80°C.

Storage/Expiration

 –20°C