Biovendor/MANF Human E. coli/RBG10209100/100 μg-免疫在线蚂蚁淘旗下平台

商品详细Biovendor/MANF Human E. coli/RBG10209100/100 μg

Biovendor/MANF Human E. coli/RBG10209100/100 μg

商品编号： RBG10209100

品牌： Biovendor-Laboratorni

市场价： ¥12000.00

美元价： 7200.00

产地：美国(厂家直采)

公司：

产品分类：其它重组蛋白

公司分类： Other_recombinant_protein

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Regulatorystatus:RUO
Type:Recombinant
Source:E.coli
Othernames:MesencephalicAstrocyte-derivedNeurotrophicFactor,ARMET,Arginine-richprotein(ARP)
Species:Human

Type

Recombinant

Description

MANFisasecretedneurotrophicfactorthatisexpressedinbrain,neuronalandcertainnon-neuronaltissues.Ithasbeenshowntopromotethesurvival,growthandfunctionofdopamine-specificneurons.MANFanditsstructuralhomologCDNFeachcontainaN-terminal,saposin-like,lipid-bindingdomain,andacarboxyl-terminaldomainthatisnothomologoustopreviouslycharacterizedproteinstructures.MANFandCDNFcanprevent6-OHDA-induceddegenerationofdopaminergicneuronsbytriggeringsurvivalpathwaysinaratexperimentalmodelofParkinson"s disease.RecombinantHumanMANFisan18.1 kDaproteinconsistingof158 aminoacids,including8 cysteineresidues.

AminoAcidSequence

LRPGDCEVCISYLGRFYQDLKDRDVTFSPATIENELIKFCREARGKENRLCYYIGATDDAATKIINEVSKPLAHHIPVEKICEKLKKKDSQICELKYDKQIDLSTVDLKKLRVKELKKILDDWGETCKGCAEKSDYIRKINELMPKYAPKAASARTDL

Source

E.coli

Purity

98%

BIOLOGicalActivity

DeterminedbyitsABIlitytostimulatetheproliferationofratC6cells. TheexpectedED₅₀ forthiseffectis15–25 µg/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

–20°C

品牌介绍

miREIA是一种基于免疫测定的新型miRNA定量方法，涉及从患者样品中分离出的miRNA与互补的生物素化DNA寡核苷酸探针杂交。然后通过固定有完美匹配的DNA / miRNA杂交体的微量滴定板固定的单克隆抗体捕获DNA / RNA杂交体。下一步是复制标准ELISA方案。该测定可在常规免疫测定设备上运行，与标准临床工作流程兼容，不需要扩增步骤，并且包括miRNA分析在内的结果可在不到三个小时的时间内获得。