Type
Recombinant
Description
MacrophageColonyStimulatingFactorMouseRecombinantproducedinE.coliisadisulfidelinkedhomodimer,non-glycosylated,polypeptidechaincontaining2×156aminoacidsandhavingatotalmolecularmassof36.4 KD.MCSFispurifiedbyproprietarychromatographictechniques.
AminoAcidSequence
MKEVSEHCSHMIGNGHLKVLQQLIDSQMETSCQIAFEFVDQEQLDDPVCYLKKAFFLVQDIIDETMRFKDNTPNANATERLQELSNNLNSCFTKDYEEQNKACVRTFHETPLQLLEKIKNFFNETKNLLEKDWNIFTKNCNNSFAKCSSRDVVTKP
Source
E.coli
Purity
Greaterthan98.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.
BIOLOGicalActivity
TheED50,calculatedbythedose-dependantstimulationoftheproliferationofmurineM-NFS-60 indicatorcellswasfound0.91 ng/ml.
Formulation
Theprotein(1 mg/ml)waslyophilizedwith0.5×PBS,pH 8.0.
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
LyophilizedM-CSFalthoughstableatroomtemperaturefor3 weeks,shouldbestoreddesiccatedbelow –18°C.UponreconstitutionMCSFshouldbestoredat4°Cbetween2–7daysandforfutureusebelow –18°C.Pleasepreventfreeze-thawcycles.
PhysicalAppearance
Sterilefilteredwhitelyophilized(freeze-dried)powder.