Type

Recombinant

Description

CXCL6,alsoknownasLIXinmice,isaCXCchemokinethatsignalsthroughtheCXCR2receptor.Itisexpressedinmonocytes,platelets,endothelialcells,andmastcells.LIXisachemoattractantforneutrophils.ThetwonaturallyoccurringvariantsofLIX,LIX1–78(GCP-2)andLIX9–78(GCP-2),contain78 and70 aminoacidresidues,respectively.LIXcontainsthefourconservedcysteineresiduespresentinCXCchemokines,andalsocontainsthe‘ELR’motifcommontoCXCchemokinesthatbindstotheCXCR1andCXCR2receptors.RecombinantMurineLIXisa9.8 kDaproteincontaining92 aminoacidresidues.

AminoAcidSequence

APSSVIAATELRCVCLTVTPKINPKLIANLEVIPAGPQCPTVEVIAKLKNQKEVCLDPEAPVIKKIIIQKILGSDKKKAKRNALAVERTASVQ

Source

E.coli

Purity

98%

BIOLOGicalActivity

DeterminedbyitsABIlitytochemoattracthumanneutrophilsusingaconcentrationrangeof10–100 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C