Type
Recombinant
Description
Total127 AA.MW:14.2 kDa(calculated).NoextraAA.UniprotKBacc.no. P07148
AminoAcidSequence
MSFSGKYQLQSQENFEAFMKAIGLPEELIQKGKDIKGVSEIVQNGKHFKFTITAGSKVIQNEFTVGEECELETMTGEKVKTVVQLEGDNKLVTTFKNIKSVTELNGDIITNTMTLGDIVFKRISKRI
Source
E.coli
Purity
Purityasdeterminedbydensitometricimageanalysis:> 90%
SDS-PAGEGel
14%SDS-PAGEseparationofHumanL-FABP
1. M.W.Marker –97,66,45,31,21,14 kDa
2.reducedandboiledsample,5μg/lane
3.non-reducedandnon-boiledsample,5μg/lane
Formulation
Filtered(0.4 μm)andlyophilizedin0.5 mg/mLin20mMTRIS,50mMNaCl,pH 7.5
Reconstitution
Add200ulofdeionizedwatertoprepareaworkingstocksolutionof0.5 mg/mLandletthelyophilizedpelletdissolvecompletely.Productisnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.
Applications
Westernblotting,ELISA
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.
QualityControlTest
BCAtodeterminequantityoftheprotein.
SDSPAGEtodeterminepurityoftheprotein.
Note
Thisproductisintendedforresearchuseonly.