Type

Recombinant

Description

LIGHTbelongstotheTNFfamilyofligands,andcansignalthroughtheherpesvirusentrymediatortypeA receptor(HVEM,TNFRSF14),LTβR,orbindtoadecoyreceptor,DcR3.Itisexpressedinsplenocytes,activatedPBL,CD8+tumorinfiltratinglymphocytes,granulocytes,andmonocytes.LIGHThastheABIlitytoactiveNF-κB,toco-stimulatetheactivationoflymphocytesandtoinduceapoptosisincertainhumantumorcells.ThehumanLIGHTgeneencodesfora240 aminoacidtypeIItransmembraneproteincontaininga37 aminoacidcytoplasmicdomain,a21 aminoacidtransmembranedomain,anda182 aminoacidextracellulardomain.RecombinantHumanLIGHThasacalculatedmassof19.3 kDa,containing177 aminoacidresidues.Duetoglycosylation,LIGHTmigratesbetween20.0–22.5 kDabySDS-PAGEundernon-reducingconditions.

AminoAcidSequence

RLGEMVTRLPDGPAGSWEQLIQERRSHEVNPAAHLTGANSSLTGSGGPLLWETQLGLAFLRGLSYHDGALVVTKAGYYYIYSKVQLGGVGCPLGLASTITHGLYKRTPRYPEELELLVSQQSPCGRATSSSRVWWDSSFLGGVVHLEAGEKVVVRVLDERLVRLRDGTRSYFGAFMV

Source

Hi-5Insectcells

Purity

95%

BIOLOGicalActivity

DeterminedbyitscytotoxiceffectonHT-29 cells(humancolonadenocarcinomacells)inthepresenceofhuman IFN-ɣ.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat-20°Cto –80°C.

Storage/Expiration

 –20°C