Type

Recombinant

Description

UniProtKBacc.no.P41160

AminoAcidSequence

MVPIQKVQDDTKTLIKTIVTRINDISHTQSVSAKQRVTGLDFIPGLHPILSLSKMDQTLAVYQQVLTSLPSQNVLQIANDLENLRDLLHLLAFSKSCSLPQTSGLQKPESLDGVLEASLYSTEVVALSRLQGSLQDILQQLDVSPEC

Source

E.coli

Purity

>98%bySDSPAGEgelanalysis

SDS-PAGEGel

14%SDS-PAGEseparationofMouseLeptin
1. M.W.Marker –14,21,31,45,66,97 kDa
2.reducedandheatedsample,5μg/lane
3.non-reducedandnon-heatedsample,5μg/lane

BIOLOGicalActivity

BiologicalactivityofMouseLeptinisperformedintwodifferentmouseobesitymodels,ob/obandNZO.Bothstrainsofmiceweretreatedviaintraperitonealinjectiononcedailyatadoseof5 µgLeptin/gmofbodyweightfor7 days.Significanteffectsonbodyweight,foodconsumption,andplasmaglucoselevelswereobservedtosaline-treatedcontrols.

Endotoxin

<1.0 EU/ug

Formulation

Filtered(0.4 μm)andlyophilizedwith0.1%TFA.

Reconstitution

Addinjectionwatertoprepareaworkingstocksolutionof1.0 mg/mLandletthelyophilizedpelletdissolvecompletely.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreat –80°C.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.

Applications

Westernblotting,ELISA

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.

LALtodeterminequantityofendotoxin.

Note

Thisproductisintendedforresearchuseonly.