Type

Recombinant

Description

LECisaCCchemokinethatcansignalthroughtheCCR8andCCR1receptors.Itisexpressedintheliver,spleen,andthymus.LECischemotactictowardsmonocytesandlymphocytes,butnotneutrophils.RecombinantHumanLECisan11.2 kDaproteincontaining97 aminoacidresidues,includingthefourconservedcysteineresiduespresentinCCchemokines.

AminoAcidSequence

QPKVPEWVNTPSTCCLKYYEKVLPRRLVVGYRKALNCHLPAIIFVTKRNREVCTNPNDDWVQEYIKDPNLPLLPTRNLSTVKIITAKNGQPQLLNSQ

Source

E.coli

Purity

98%

BIOLOGicalActivity

DeterminedbyitsABIlitytochemoattracttotalhumanmonocytesusingaconcentrationrangeof10–100 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C