Type

Recombinant

Description

LAG-1 isCCchemokinethatsignalsthroughtheCCR5receptor.LAG-1 isidenticaltoMIP-1β(ACTIIisotype)exceptfortwoaminoacidsubstitutions;arginineforhistidineatposition22 andserineforglycineatposition47 ofthematureprotein.LAG-1 chemoattractsmonocytes,andexhibitsactivityasanHIV-suppressivefactor.RecombinantHumanLAG-1 isa7.7 kDaproteincontaining69 aminoacidresidues.

AminoAcidSequence

APMGSDPPTACCFSYTARKLPRNFVVDYYETSSLCSQPAVVFQTKRSKQVCADPSESWVQEYVYDLELN

Source

E.coli

Purity

98%

BIOLOGicalActivity

Determinedbyit"s ABIlitytochemoattractCCR5transfectedcellsusingaconcentrationof0.1–10.0 ng/ml

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C