Type

Recombinant

Description

Total522 AA.Mw:59,5 kDa(calculated)C-terminalhis-tag(6 extraAA)UniProtentryQ9UEF7–2,Isoform2(secreted)

AminoAcidSequence

EPGDGAQTWARFSRPPAPEAAGLFQGTFPDGFLWAVGSAAYQTEGGWQQHGKGASIWDTFTHHPLAPPGDSRNASLPLGAPSPLQPATGDVASDSYNNVFRDTEALRELGVTHYRFSISWARVLPNGSAGVPNREGLRYYRRLLERLRELGVQPVVTLYHWDLPQRLQDAYGGWANRALADHFRDYAELCFRHFGGQVKYWITIDNPYVVAWHGYATGRLAPGIRGSPRLGYLVAHNLLLAHAKVWHLYNTSFRPTQGGQVSIALSSHWINPRRMTDHSIKECQKSLDFVLGWFAKPVFIDGDYPESMKNNLSSILPDFTESEKKFIKGTADFFALCFGPTLSFQLLDPHMKFRQLESPNLRQLLSWIDLEFNHPQIFIVENGWFVSGTTKRDDAKYMYYLKKFIMETLKAIKLDGVDVIGYTAWSLMDGFEWHRGYSIRRGLFYVDFLSQDKMLLPKSSALFYQKLIEKNGFPPLPENQPLEGTFPCDFAWGVVDNYIQVSQLTKPISSLTKPYHHHHHHH

Source

CHO

Purity

Morethan90%asdeterminedbydensitometricimageanalysisofcoomasiestainedSDS-PAGE

SDS-PAGEGel

12%SDS-PAGEseparationofHumanKlothoprotein
1. M.W.Marker –14,21,31,45,66,97 kDa
2.reducedandboiledsample,5μg/lane
3.non-reducedandnon-boiledsample,5μg/lane

Endotoxin

<1.0 EU/ug

Formulation

Filtered(0,4 μm)andlyophilizedin0.5 mg/mLin0.08 Mphosphatebuffer,0.1 MNaCl,pH 7.2

Reconstitution

Adddeionizedwatertoprepareaworkingstocksolutionof0.5 mg/mLandletthelyophilizedpelletdissolvecompletely.Productisnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.

Applications

Westernblotting,ELISA,Cellcultureand/oranimalstudies,Immunoassays

Shipping

Attemperature2 –8°C.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.

LALtodeterminequantityofendotoxin.