Type

Recombinant

Description

KLF4isamemberoftheKruppel-likefactor(KLF)familyofzincfingertranscriptionfactors.Membersofthisfamilyshare3 contiguousC2H2-typezincfingersatthecarboxylterminusthatcomprisetheDNA-bindingdomain.KLF4ishighlyexpressedinskinandgutepithelialtissues,butisalsofoundinvariousothercellsandtissues,includingvascularendothelialcells,lymphocytes,lung,andtestis.Itisanimportantregulatorofthecellcycle,transcription,andcelldifferentiation.TogetherwithSox2,Oct4,andcMyc,KLF4caninducethereprogrammingofprimaryhumanfibroblaststoapluripotentstate.KLF4andothertranscriptionfactorscanbeintroducedintocellsbyDNAtransfection,viralinfection,ormicroinjection.ProteintransductionusingTATfusionproteinsrepresentsanalternativemethodologyforintroducingtranscriptionfactorsintoprimary,aswellastransformed,cells.RecombinantHumanKLF4-TATisa51.7 kDaproteincontaining483 aminoacidresidues,includinga13-residueC-terminalTATpeptide.

AminoAcidSequence

MAVSDALLPSFSTFASGPAGREKTLRQAGAPNNRWREELSHMKRLPPVLPGRPYDLAAATVATDLESGGAGAACGGSNLAPLPRRETEEFNDLLDLDFILSNSLTHPPESVAATVSSSASASSSSSPSSSGPASAPSTCSFTYPIRAGNDPGVAPGGTGGGLLYGRESAPPPTAPFNLADINDVSPSGGFVAELLRPELDPVYIPPQQPQPPGGGLMGKFVLKASLSAPGSEYGSPSVISVSKGSPDGSHPVVVAPYNGGPPRTCPKIKQEAVSSCTHLGAGPPLSNGHRPAAHDFPLGRQLPSRTTPTLGLEEVLSSRDCHPALPLPPGFHPHPGPNYPSFLPDQMQPQVPPLHYQELMPPGSCMPEEPKPKRGRRSWPRKRTATHTCDYAGCGKTYTKSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKHTGHRPFQCQKCDRAFSRSDHLALHMKRHFGGYGRKKRRQRRR

Source

HEK293cells

Purity

90%

BIOLOGicalActivity

Datanotavailable.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C