Type

Recombinant

Description

IP-10 isaCXCchemokinethatsignalsthroughtheCXCR3receptor.IP-10 selectivelychemoattractsTh1lymphocytesandmonocytes,andinhibitscytokine-stimulatedhematopoieticProgenitorcellproliferation.Additionally,itisangiostaticandmitogenicforvascularsmoothmusclecells.RecombinantHumanIP-10 isan8.6 kDaproteinconsistingof77 aminoacids,includingthefourconservedcysteineresiduespresentinCXCchemokines.

AminoAcidSequence

VPLSRTVRCTCISISNQPVNPRSLEKLEIIPASQFCPRVEIIATMKKKGEKRCLNPESKAIKNLLKAVSKERSKRSP

Source

E.coli

Purity

98%

BIOLOGicalActivity

DeterminedbyitsABIlitytochemoattracthumanT-Lymphocytesusingaconcentrationof10–50 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C