Type
Recombinant
Description
IL-9 isanimmunoregulatorycytokineproducedbyIL-2 activatedTh4lymphocytes.IL-9 enhancestheproliferationofTlymphocytes,mastcells,erythroidprecursorcellsandmegakaryoblasticleukemiacelllines.Over-expressionofIL-9 hasbeenimplicatedinthepathogenesisofanaplasticlymphomaandHodgkin’s disease.WhereasmurineIL-9 canfunctiononhumancells,humanIL-9 isinactiveonmousecells.RecombinantHumanIL-9 isa14.0 kDaproteinof127 aminoacidresidues,including10 cysteineresiduesthatarefullyconservedbetweenthehumanmurineproteins.
AminoAcidSequence
MQGCPTLAGILDINFLINKMQEDPASKCHCSANVTSCLCLGIPSDNCtrpCFSERLSQMTNTTMQTRYPLIFSRVKKSVEVLKNNKCPYFSCEQPCNQTTAGNALTFLKSLLEIFQKEKMRGMRGKI
Source
E.coli
Purity
98%
BIOLOGicalActivity
TheED50 asdeterminedbythedose-dependentproliferationofhumanM07ecellswas≤0.2 ng/ml,correspondingtoaspecificactivityof≥5×106 units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
