Type

Recombinant

Description

IL-8 isaproinflammatoryCXCchemokinethatcansignalthroughtheCXCR1andCXCR2receptors.Itissecretedbymonocytesandendothelialcells.IL-8 chemoattractsandactivatesneutrophils.RecombinantHumanIL-8(endothelial-derived)isan8.9 kDaproteincontaining77 aminoacidresidues.

AminoAcidSequence

AVLPRSAKELRCQCIKTYSKPFHPKFIKELRVIESGPHCANTEIIVKLSDGRELCLDPKENWVQRVVEKFLKRAENS

Source

E.coli

Purity

98%

BIOLOGicalActivity

DeterminedbyitsABIlitytochemoattracthumanperipheralbloodneutrophilsusingaconcentrationrangeof25.0–150.0 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C