Type
Recombinant
Description
IL-6 isapleiotropiccytokinethatplaysanimportantroleinhostdefensebyregulatingimmuneandinflammatoryresponses.ProducedbyTcells,monocytes,fibroblasts,endothelialcellsandkeratinocytes,IL-6 hasdiverseBIOLOGicalfunctions.ItstimulatesBcelldifferentiationandantibodyproduction,synergizeswithIL-3 inmegakaryocytedevelopmentandplateletproduction,inducesexpressionofhepaticacute-phaseproteins,andregulatesbonemetabolism.IL-6 signalsthroughtheIL-6 receptorsystemthatconsistsoftwochains,IL-6Rαandgp130.MurineIL-6 isinactiveonhumancells,whilebothhumanandmurineareequallyactiveonmurinecells.RecombinantRatIL-6 isa21.7 kDaproteincontaining188 aminoacidresidues.
AminoAcidSequence
MFPTSQVRRGDFTEDTTHNRPVYTTSQVGGLITYVLREILEMRKELCNGNSDCMNSDDALSENNLKLPEIQRNDGCFQTGYNQEICLLKICSGLLEFRFYLEFVKNNLQDNKKDKARVIQSNTETLVHIFKQEIKDSYKIVLPTPTSNALLMEKLESQKEWLRTKTIQLILKALEEFLKVTMRSTRQT
Source
E.coli
Purity
98%
BiologicalActivity
TheED50asdeterminedbythedose-dependentstimulationoftheproliferationofIL-6-dependentmurine7TD1cellsis<0.01 ng/ml,correspondingtoaspecificactivityof>1×108units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
