Type
Recombinant
Description
IL-5 isahematopoieticgrowthfactorthatstimulatestheproliferationandactivationofeosinophils.Producedbymastcells,Tcells,andeosinophils,IL-5 playsanimportantroleininducingcell-mediatedimmunityagainstparasiticinfectionsandcertaintumors.ElevatedlevelsofIL-5 leadtoEosinophilia,whichmayresultintheinductionofasthmaandotherallergicdiseases.HumanandmurineIL-5 iscross-speciesreactive.RecombinantHumanIL-5 isa26.5 kDadisulfide-linkedhomodimericproteincontainingtwo116 aminoacidchains.
AminoAcidSequence
MIPTEIPTSALVKETLALLSTHRTLLIANETLRIPVPVHKNHQLCTEEIFQGIGTLESQTVQGGTVERLFKNLSLIKKYIDGQKKKCGEERRRVNQFLDYLQEFLGVMNTEWIIES
Source
E.coli
Purity
98%
BIOLOGicalActivity
TheED50 asdeterminedbythedose-dependentproliferationofTF-1 cellswas≤0.15 ng/ml,correspondingtoaspecificactivityof≥6.6×106 units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
