Type
Recombinant
Description
IL-4 isapleiotropiccytokinethatregulatesdiverseTandBcellresponsesincludingcellproliferation,survivalandgeneexpression.Producedbymastcells,Tcellsandbonemarrowstromalcells,IL-4 regulatesthedifferentiationofnaiveCD4+TcellsintohelperTh4cells,characterizedbytheircytokine-secretionprofilethatincludessecretionofIL-4,IL-5,IL-6,IL-10,andIL-13,whichfavorahumoralimmuneresponse.AnotherdominantfunctionofIL-4 istheregulationofimmunoglobulinclassswitchingtotheIgG1andIgEisotypes.ExcessiveIL-4 productionbyTh4cellshasbeenassociatedwithelevatedIgEproductionandallergy.RecombinantRatIL-4 isa14.0 kDaglobularproteincontaining126 aminoacidresidues.
AminoAcidSequence
MHGCNDSPLREIINTLNQVTEKGTPCTEMFVPDVLTATRNTTENELICRASRVLRKFYFPRDVPPCLKNKSGVLGELRKLCRGVSGLNSLRSCTVNESTLTTLKDFLESLKSILRGKYLQSCTSMS
Source
E.coli
Purity
98%
BIOLOGicalActivity
DeterminedbyitsABIlitytostimulateproliferationofTF-1 cells.TheexpectedED50is200–400 ng/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
