Type
Recombinant
Description
IL-4 isapleiotropiccytokinethatregulatesdiverseTandBcellresponsesincludingcellproliferation,survivalandgeneexpression.Producedbymastcells,Tcellsandbonemarrowstromalcells,IL-4 regulatesthedifferentiationofnaiveCD4+TcellsintohelperTh4cells,characterizedbytheircytokine-secretionprofilethatincludessecretionofIL-4,IL-5,IL-6,IL-10,andIL-13,whichfavorahumoralimmuneresponse.AnotherdominantfunctionofIL-4 istheregulationofimmunoglobulinclassswitchingtotheIgG1andIgEisotypes.ExcessiveIL-4 productionbyTh4cellshasbeenassociatedwithelevatedIgEproductionandallergy.RecombinantMurineIL-4 isa13.5 kDaglobularproteincontaining121 aminoacidresidues.
AminoAcidSequence
MHIHGCDKNHLREIIGILNEVTGEGTPCTEMDVPNVLTATKNTTESELVCRASKVLRIFYLKHGKTPCLKKNSSVLMELQRLFRAFRCLDSSISCTMNESKSTSLKDFLESLKSIMQMDYS
Source
E.coli
Purity
98%
BIOLOGicalActivity
TheED50wasdeterminedbythedose-dependentproliferationofmurineHT-2 cellsis≤2.0 ng/ml,correspondingtoaspecificactivityof≥5×105units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
