Type
Recombinant
Description
IL-3 isahematopoieticgrowthfactorthatpromotesthesurvival,differentiationandproliferationofcommittedProgenitorcellsofthemegakaryocyte,granulocyte-macrophage,erythroid,eosinophil,basophilandmastcelllineages.ProducedbyTcells,mastcellsandeosinophils,IL-3 enhancesthrombopoiesis,phagocytosis,andantibody-mediatedcellularcytotoxicity.ItsABIlitytoactivatemonocytessuggeststhatIL-3 mayhaveadditionalimmunoregulatoryroles.ManyoftheIL-3 activitiesdependuponco-stimulationwithothercytokines.IL-3 isaspecies-specific,variablyglycosylatedcytokine.RecombinantRatIL-3βisa16.3 kDaglobularproteincontaining144 aminoacidresidues.
AminoAcidSequence
MISDRGSDAHHLLRTLDCRTIALEILVKLPYPQVSGLNNSDDKANLRNSTLRRVNLDEFLKSQEEFDSQDTTDIKSKLQKLKCCIPAAASDSVLPGVYNKDLDDFKKKLRFYVIHLKDLQPVSVSRPPQPTSSSDNFRPMTVEC
Source
E.coli
Purity
98%
BIOLOGicalActivity
TheED50wasdeterminedbythedose-dependentstimulationoftheproliferationofthymidineuptakebymurineMC-9 cellsis<10 ng/ml,correspondingtoaspecificactivityof>1.0×105units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
