Type

Recombinant

Description

TheIL-1 familyiscomprisedof11 structurallyrelatedligands,includingtherecentlyre-namedIL-36RA(IL-1F5),IL-36α(IL-1F6),IL-36β(IL-1F8),andIL-36γ(IL-1F9).TheinteractionofIL-36 ligandswiththeIL-1Rrp2receptor(IL-1R6)caninducevariousactivities,includingdendriticcellmaturationandactivation.IL-36RAcanantagonizetheNF-κBsignalinginducedbyeitherIL-36α,βorγbybindingtotheIL-1Rrp2receptorinamannerthatpreventstheinitiationoffunctionalsignaling.RecombinantHumanIL-36RAisanE.coli-derived17 kDaproteincontaining154 aminoacidresidues.

AminoAcidSequence

VLSGALCFRMKDSALKVLYLHNNQLLAGGLHAEKVIKGEEISVVPNRALDASLSPVILGVQGGSQCLSCGTEKGPILKLEPVNIMELYLGAKESKSFTFYRRDMGLTSSFESAAYPGWFLCTSPEADQPVRLTQIPEDPAWDAPITDFYFQQCD

Source

E.coli

Purity

98%

BIOLOGicalActivity

MeasuredbyitsABIlitytoinhibitIL-36ɣ;inducedIL-6 productionbyhuman PBMCs.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C