Type

Recombinant

Description

TheIL-1 familyiscomprisedof11 structurallyrelatedligands,includingrecentlyre-namedIL-36α(IL-1F6),β(IL-1F8)andγ(IL-1F9).IL-36βishighlyexpressedinpsoriaticplaques,andatlowerlevelsinvariousothertissues.IL-36βsignalsthroughtheIL-1Rrp2(IL-1R6)receptor,whichisprimarilyexpressedoncertaindendriticcells.TheinteractionoftheIL-1Rrp2receptorwithIL-36 ligandsinducesdendriticcellmaturationandactivation.IL-36βalsofunctionsasanagoNISTofNF-κB,andstimulatestheproductionofpro-inflammatoryproteins,includingIL-6,IL-8,BD-2,andBD-3. RecombinantHumanIL-36βisa17.3 kDaproteincontaining154 aminoacidresidues.

AminoAcidSequence

MREAAPKSYAIRDSRQMVWVLSGNSLIAAPLSRSIKPVTLHLIACRDTEFSDKEKGNMVYLGIKGKDLCLFCAEIQGKPTLQLKEKNIMDLYVEKKAQKPFLFFHNKEGSTSVFQSVSYPGWFIATSTTSGQPIFLTKERGITNNTNFYLDSVE

Source

E.coli

Purity

98%

BIOLOGicalActivity

MeasuredbyitsABIlitytoinduceIL-8 productionbyhuman PBMCs.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C