Type

Recombinant

Description

IL-34 isahomodimericcytokinethatisexpressedinarangeoftissuesthatincludespleen,heart,brain,liver,kidney,lung,ovary,thymus,testis,smallintestine,prostateandcolon.IL-34 isaligandforcolony-stimulatingfactor-1 receptor(CSF1R),whichalsobindstoCSF-1. ItspecificallybindsCD14+monocytes,promotessurvivalandproliferationofhumanperipheralbloodmonocytes,andstimulatesmacrophagecolonyformationbyhumanbonemarrowcells.IL-34,likehumanCSF-1,stimulatesphosphorylationofMAPK1/ERK2andMAPK3/ERK1.RecombinantHumanIL-34 isa52.5 kDahomodimericglycoproteinconsistingof460 aminoacidresidues,includingaC-terminalHis-Tag.

AminoAcidSequence

NEPLEMWPLTQNEECTVTGFLRDKLQYRSRLQYMKHYFPINYKISVPYEGVFRIANVTRLQRAQVSERELRYLWVLVSLSATESVQDVLLEGHPSWKYLQEVETLLLNVQQGLTDVEVSPKVESVLSLLNAPGPNLKLVRPKALLDNCFRVMELLYCSCCKQSSVLNWQDCEVPSPQSCSPEPSLQYAATQLYPPPPWSPSSPPHSTGSVRPVRAQGEGLLPHHHHHHHH

Source

HEK293cells

Purity

95%

BIOLOGicalActivity

MeasuredbyitsABIlitytoinducesecretionofMCP-1 from PBMCs.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C