Type
Recombinant
Description
IL-33 isaproinflammatoryproteinthatsharesstructuralandfunctionalcharacteristicswiththeIL-1 cytokinefamily.ItbindsandsignalsthroughtheIL-1RL1/ST2receptor,toactivateNF-κBandMAPkinases.IL-33 inducesproductionofTH2cellrelatedcytokines,includingIL-4,IL-5 andIL-13,andexertsmultipleinflammationrelatedbioactivities.RecombinantMurineIL-33 isa17.5 kDaproteincontaining158 aminoacidresidues.
AminoAcidSequence
SIQGTSLLTQSPASLSTYNDQSVSFVLENGCYVINVDDSGKDQEQDQVLLRYYESPCPASQSGDGVDGKKLMVNMSPIKDTDIWLHANDKDYSVELQRGDVSPPEQAFFVLHKKSSDFVSFECKNLPGTYIGVKDNQLALVEEKDESCNNIMFKLSKI
Source
E.coli
Purity
98%
BIOLOGicalActivity
TheED50wasdeterminedbythedose-dependentstimulationoftheproliferationofmurineD10Scellsis≤0.5 ng/ml,correspondingtoaspecificactivityof≥2×106units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
