Type

Recombinant

Description

IL-31 isaTcell-derivedcytokinethatsharesseveralstructuralandfunctionalcharacteristicswithIL-6,OncostatinM,LIF,andCardiotrophin-1. ItsignalsthroughareceptorcomplexcomprisedofGPL(alsoknownasgp130-likereceptororIL-31RA)andOncostatin-Mreceptor(OSMR).GPL/OSMRsignalingisastrongactivatorofSTAT3andSTAT5,andcanalsoactivateSTAT1,JAK1,andJAK2signalingpathways.IL-31-regulatedimmuneresponseshavebeenimplicatedinskinphysiologyandinflammatoryskindiseases.RecombinantMurineIL-31 isa15.8 kDaproteincontaining141 aminoacidresidues.

AminoAcidSequence

MTCSLSFGAPISKEDLRTTIDLLKQESQDLYNNYSIKQASGMSADESIQLPCFSLDREALTNISVIIAHLEKVKVLSENTVDTSWVIRWLTNISCFNPLNLNISVPGNTDESYDCKVFVLTVLKQFSNCMAELQAKDNTTC

Source

E.coli

Purity

98%

BIOLOGicalActivity

Datanotavailable.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C