Type

Recombinant

Description

IL-20 isamemberoftheIL-10 familyofregulatorycytokines,whichincludesIL-10,IL-19,IL-20,IL-22,IL-24 andIL-26. Membersofthisfamilysharepartialhomologyintheiraminoacidsequences,buttheyaredissimilarintheirBIOLOGicalfunctions.IL-20 isahematopoieticgrowthfactorcapableofstimulatingcolonyformationbyCD34+multipotentialProgenitors,butnotbyotherprogenitorcells.IL-20 signalsthroughareceptorsystemcomposedoftypeI IL-20RαandtypeIIIL-20Rβ.Over-expressionofIL-20 inkeratinocytesexpressingbothreceptorsubunitshasbeenimplicatedintheinductionofinflammatoryskindisease.RecombinantHumanIL-20 isa35.2 kDahomodimericproteinconsistingoftwo153 aminoacidpolypeptidechains.

AminoAcidSequence

MLKTLNLGSCVIATNLQEIRNGFSEIRGSVQAKDGNIDIRILRRTESLQDTKPANRCCLLRHLLRLYLDRVFKNYQTPDHYTLRKISSLANSFLTIKKDLRLCHAHMTCHCGEEAMKKYSQILSHFEKLEPQAAVVKALGELDILLQWMEETE

Source

E.coli

Purity

98%

BiologicalActivity

DeterminedbyitsABIlitytoactivateSTATfollowingreceptorligandinteraction.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.5–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C