Type

Recombinant

Description

IL-2 isapowerfulimmunoregulatorylymphokineproducedbyT-cellsinresponsetoantigenicormitogenicstimulation.IL-2/IL-2RsignalingisrequiredforT-cellproliferationandotherfundamentalfunctionsthatareessentialfortheimmuneresponse.IL-2 stimulatesgrowthanddifferentiationofB-cells,NKcells,lymphokine-activatedkillercells,monocytes,macrophagesandoligodendrocytes.RecombinantRatIL-2 isa15.3 kDaproteincontaining134 aminoacidresidues.

AminoAcidSequence

MAPTSSPAKETQQHLEQLLLDLQVLLRGIDNYKNLKLPMMLTFKFYLPKQATELKHLQCLENELGALQRVLDLTQSKSFHLEDAGNFISNIRVTVVKLKGSENKFECQFDDEPATVVEFLRRWIAIAQSIISTM

Source

E.coli

Purity

98%

BIOLOGicalActivity

TheED₅₀asdeterminedbythedose-dependentstimulationoftheproliferationofmurineCTLL-2 cellsis0.4 ng/ml,correspondingtoaspecificactivityof2.5×10⁶units/mg.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C