Type
Recombinant
Description
IL-2 isapowerfulimmunoregulatorylymphokineproducedbyT-cellsinresponsetoantigenicormitogenicstimulation.IL-2/IL-2RsignalingisrequiredforT-cellproliferationandotherfundamentalfunctionsthatareessentialfortheimmuneresponse.IL-2 stimulatesgrowthanddifferentiationofB-cells,NKcells,lymphokine-activatedkillercells,monocytes,macrophagesandoligodendrocytes.RecombinantHumanIL-2 isa15.5 kDaprotein,containing134 aminoacidresiduesincludingoneintrachaindisulfide bond.
AminoAcidSequence
MAPTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLT
Source
E.coli
Purity
98%
BIOLOGicalActivity
TheED50 asdeterminedbythedose-dependentstimulationofmurineCTLL-2 cellsis≤0.1 ng/ml,correspondingtoaspecificactivityof≥1×107 units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstitutein100 mMAceticAcidtoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
