Type
Recombinant
Description
Interleukin-1 receptorantagoNIST(IL-1RA)isanaturally-occurring,inflammatory-inhibitorprotein.ItinhibitstheactivityofIL-1αandIL-1βbycompetitivelyblockingtheirbindingtotypeI andtypeIIreceptors.IL-1RAisproducedbycornealepithelialcells,monocytes,neutrophils,macrophages,andfibroblasts.Therapeutically,IL-1RAmayhelpinthetreatmentofsepsis,cachexia,rheumatoidarthritis,chronicmyelogenousleukemia,asthma,psoriasis,andinflammatoryboweldisease.RecombinantHumanIL-1RAisa17.2 kDaproteinconsistingof153 aminoacidresidues.
AminoAcidSequence
MRPSGRKSSKMQAFRIWDVNQKTFYLRNNQLVAGYLQGPNVNLEEKIDVVPIEPHALFLGIHGGKMCLSCVKSGDETRLQLEAVNITDLSENRKQDKRFAFIRSDSGPTTSFESAACPGWFLCTAMEADQPVSLTNMPDEGVMVTKFYFQEDE
Source
E.coli
Purity
98%
BIOLOGicalActivity
DeterminedbyitsABIlitytoinhibittheIL-1αstimulationofmurineD10Scell.TheexpectedED50 is20–40ng/mlinthepresenceof50 pg/mlof IL-1α.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
