Type
Recombinant
Description
IL-1bisaproinflammatorycytokineproducedinavarietyofcells,includingmonocytes,tissuemacrophages,keratinocytes,andotherepithelialcells.BothIL-1aandIL-1bbindtothesamereceptorandhavesimilar,ifnotidentical,BIOLOGicalproperties.Thesecytokineshaveabroadrangeofactivities,includingthestimulationofthymocyteproliferationbyinducingIL-2 release,B-cellmaturationandproliferation,mitogenicFGF-likeactivity,and,thereleaseofprostaglandinandCollagenasefromsynovialcells.However,whereasIL-1bisasecretedcytokine,IL-1aispredominantlyacell-associatedcytokine.RecombinantRatIL-1bisa17.4 kDaproteincontaining153 aminoacidresidues.
AminoAcidSequence
MVPIRQLHCRLRDEQQKCLVLSDPCELKALHLNGQNISQQVVFSMSFVQGETSNDKIPVALGLKGLNLYLSCVMKDGTPTLQLESVDPKQYPKKKMEKRFVFNKIEVKTKVEFESAQFPNWYISTSQAEHRPVFLGNSNGRDIVDFTMEPVSS
Source
E.coli
Purity
98%
BiologicalActivity
TheED50wasdeterminedbythedose-dependentstimulationofmurineD10Scellsis0.1 ng/ml,correspondingtoaspecificactivityof1×107units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
