Type
Recombinant
Description
IL-1βisaproinflammatorycytokineproducedinavarietyofcells,includingmonocytes,tissuemacrophages,keratinocytes,andotherepithelialcells.BothIL-1αandIL-1βbindtothesamereceptorandhavesimilar,ifnotidentical,BIOLOGicalproperties.ThesecytokineshaveabroadrangeofactivitiesincludingthestimulationofthymocyteproliferationbyinducingIL-2 release,B-cellmaturationandproliferation,mitogenicFGF-likeactivity,andthereleaseofprostaglandinandCollagenasefromsynovialcells.However,whereasIL-1βisasecretedcytokine,IL-1αispredominantlyacell-associatedcytokine.RecombinantHumanIL-1βisa17.3 kDaproteincontaining153 aminoacidresidues.
AminoAcidSequence
APVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS
Source
E.coli
Purity
98%
BiologicalActivity
TheED50 asdeterminedbythedose-dependentstimulationofthymidineuptakebymurineD10Scellsis≤1.0 pg/ml,correspondingtoaspecificactivityof≥1×109 units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8oCforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
