Type
Recombinant
Description
IL-1αisanon-secreted,proinflammatorycytokineproducedinavarietyofcells,includingmonocytes,tissuemacrophages,keratinocytes,andotherepithelialcells.BothIL-1αandIL-1βbindtothesamereceptorandhavesimilar,ifnotidentical,BIOLOGicalproperties.ThesecytokineshaveabroadrangeofactivitiesincludingthestimulationofthymocyteproliferationbyinducingIL-2 release,B-cellmaturationandproliferation,mitogenicFGF-likeactivityandthereleaseofprostaglandinandCollagenasefromsynovialcells.However,whereasIL-1βisasecretedcytokine,IL-1αispredominantlyacell-associatedcytokine.RecombinantHumanIL-1αisan18.0 kDaproteincontaining159 aminoacidresidues.
AminoAcidSequence
SAPFSFLSNVKYNFMRIIKYEFILNDALNQSIIRANDQYLTAAALHNLDEAVKFDMGAYKSSKDDAKITVILRISKTQLYVTAQDEDQPVLLKEMPEIPKTITGSETNLLFFWETHGTKNYFTSVAHPNLFIATKQDYWVCLAGGPPSITDFQILENQA
Source
E.coli
Purity
98%
BiologicalActivity
TheED50 asdeterminedbythedose-dependentstimulationofmurineD10Scellsis≤0.001 ng/ml,correspondingtoaspecificactivityof≥1×109 units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat–20°Cto–80°C.
Storage/Expiration
–20°C
