Type

Recombinant

Description

IL-19 belongstotheIL-10 familyofregulatorycytokines,whichincludesIL-10,IL-19,IL-20,IL-22,IL-24 andIL-26. Membersofthisfamilysharepartialhomologyintheiraminoacidsequences,buttheyaredissimilarintheirBIOLOGicalfunctions.PreliminarydatasuggeststhatIL-19 isaproinflammatorycytokine,becauseitup-regulatesIL-6 andTNF-α,andinducesapoptosisthroughTNF-α.IL-19 signalsthroughthetypeI IL-20R.HumanandmurineIL-19 share71%aminoacidsequenceidentity.RecombinantHumanIL-19 isa35.8 kDahomodimeroftwo154 aminoacidchains.Insolution,IL-19 existspredominantlyasanon-disulfide-linked dimer.

AminoAcidSequence

MLRRCLISTDMHHIEESFQEIKRAIQAKDTFPNVTILSTLETLQIIKPLDVCCVTKNLLAFYVDRVFKDHQEPNPKILRKISSIANSFLYMQKTLRQCQEQRQCHCRQEATNATRVIHDNYDQLEVHAAAIKSLGELDVFLAWINKNHEVMSSA

Source

E.coli

Purity

98%

BiologicalActivity

DeterminedbyitsABIlitytoactivateSTATfollowingreceptor-ligandinteraction.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstitutein10 mMSodiumCitrate,pH3.0 toaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C