Type

Recombinant

Description

IL-17F,amemberoftheIL-17 familyofstructurallyrelatedcytokines,hasbeenshowntostimulatetheproliferationandactivationofT-cellsandPBMCs.IL-17Falsoregulatescartilagematrixturnoverandinhibitsangiogenesis.ThematurehumanIL-17Fisahomodimericproteinwithatotalweightof30.1 kDa,consistingoftwo133 aminoacidresiduechains.E.coli-derivedRecombinantHumanIL-17FisaBIOLOGicallyactive,non-glycosylated,disulfide-linkedhomodimericproteincontaining268 aminoacids(30.1 kDa),includingN-terminalmethionineresidues.

AminoAcidSequence

MRKIPKVGHTFFQKPESCPPVPGGSMKLDIGIINENQRVSMSRNIESRSTSPWNYTVTWDPNRYPSEVVQAQCRNLGCINAQGKEDISMNSVPIQQETLVVRRKHQGCSVSFQLEKVLVTVGCTCVTPVIHHVQ

Source

E.coli

Purity

98%

BiologicalActivity

MeasuredbyitsABIlitytoinduceIL-6 productionbyNHDF cells.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C