Type

Recombinant

Description

IL-17Eisadisulfide-linkedhomodimeroftwo145 aminoacidpolypeptidechains.ItbelongstotheIL-17 familyofstructurally-relatedcytokinesthatshareahighlyconservedC-terminalregion,butdifferfromoneanotherintheirN-terminalregionsandintheirdistinctBIOLOGicalroles.Thesixknownmembersofthisfamily,IL-17AthroughIL-17F,aresecretedashomodimers.IL-17EstimulatessecretionofIL-8,andinducesactivationofthetranscriptionfactorNF-κBincellsthatexpresstheIL-17BRreceptor.RecombinantHumanIL-17Eisa33.8 kDadisulfide-linkedhomodimeroftwo146 aminoacidpolypeptidechains.

AminoAcidSequence

MYSHWPSCCPSKGQDTSEELLRWSTVPVPPLEPARPNRHPESCRASEDGPLNSRAISPWRYELDRDLNRLPQDLYHARCLCPHCVSLQTGSHMDPRGNSELLYHNQTVFYRRPCHGEKGTHKGYCLERRLYRVSLACVCVRPRVMG

Source

E.coli

Purity

98%

BiologicalActivity

DeterminedbyitsABIlitytoinduceIL-8 inhumanPBMCsusingaconcentrationrangeof10–100 ng.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C