Type

Recombinant

Description

IL-17Bisadisulfide-linkedhomodimeroftwo161 aminoacidpolypeptidechains.ItbelongstotheIL-17 familyofstructurally-relatedcytokinesthatshareahighlyconservedC-terminalregion,butdifferfromoneanotherintheirN-terminalregionsandintheirdistinctBIOLOGicalroles.Thesixknownmembersofthisfamily,IL-17AthroughIL-17F,aresecretedashomodimers.IL-17BisexpressedbyT-cells,andhasbeenshowntostimulatereleaseofTNF-αandIL-1βfromcellsofthemonocytelineage.RecombinantHumanIL-17Bisa36.6 kDanon-disulfide-linkedhomodimeroftwo161 aminoacidpolypeptidechains.

AminoAcidSequence

MQPRSPKSKRKGQGRPGPLAPGPHQVPLDLVSRMKPYARMEEYERNIEEMVAQLRNSSELAQRKCEVNLQLWMSNKRSLSPWGYSINHDPSRIPVDLPEARCLCLGCVNPFTMQEDRSMVSVPVFSQVPVRRRLCPPPPRTGPCRQRAVMETIAVGCTCIF

Source

E.coli

Purity

98%

BiologicalActivity

DeterminedbyitsABIlitytoinduceIL-8 inhumanPBMCsusingaconcentrationrangeof10ng-100ng.PleaseNote:ResultsmayvarywithPBMCdonors.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C