Type
Recombinant
Description
IL-15 isanimmunomodulatingcytokinethatstimulatestheproliferationofTlymphocytesandsharesmanyBIOLOGicalpropertieswithIL-2. IL-15 exertsitsbiologicalactivitiesprimarilyonTcells.Itisalsoessentialinthedevelopment,survivalandactivationofNKcells.IncreasedexpressionofIL-15 hasbeenlinkedtorheumatoidarthritis,inflammatoryboweldisease,anddiseasesaffiliatedwithretrovirusesHIVandHTLV-I.HumanIL-15 isbiologicallyactiveonmousecellsasmeasuredbythedose-dependentstimulationoftheproliferationofmouseCTLLcells.RecombinantRatIL-15 isa13.5 kDaproteinconsistingof115 aminoacidresidues.
AminoAcidSequence
MNWIDVRYDLEKIESLIQFIHIDTTLYTDSDFHPSCKVTAMNCFLLELQVILHEYSNMTLNETVRNVLYLANSTLSSNKNVIESGCKECEELEERNFTEFLQSFIHIVQMFINTS
Source
E.coli
Purity
98%
BiologicalActivity
TheED50asdeterminedbythedose-dependantstimulationoftheproliferationofmurineCTLL-2 cellsis10 ng/ml,correspondingtoaspecificactivityof1×105units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
