Type
Recombinant
Description
IL-13 isanimmunoregulatorycytokineproducedprimarilybyactivatedTh4cells,andalsobymastcellsandNKcells.TargeteddeletionofIL-13 inmiceresultedinimpairedTh4celldevelopmentandindicatedanimportantroleforIL-13 intheexpulsionofgastrointestinalparasites.IL-13 exertsanti-inflammatoryeffectsonmonocytesandmacrophagesanditinhibitstheexpressionofinflammatorycytokinessuchasIL-1β,TNF-α,IL-6 andIL-8. IL-13 hasalsobeenshowntoenhanceBcellproliferationandtoinduceisotypeswitching,resultinginincreasedproductionofIgE.BlockingofIL-13 activityinhibitsthepathophysiologyofasthma.HumanandmurineIL-13 arecross-speciesreactive.RecombinantMurineIL-13 isa12.3 kDaproteinconsistingof111 aminoacidresidues.
AminoAcidSequence
MPVPRSVSLPLTLKELIEELSNITQDQTPLCNGSMVWSVDLAAGGFCVALDSLTNISNCNAIYRTQRILHGLCNRKAPTTVSSLPDTKIEVAHFITKLLSYTKQLFRHGPF
Source
E.coli
Purity
98%
BIOLOGicalActivity
TheED50asdeterminedbythedose-dependentproliferationofTF-1 cellswas4.0 ng/ml,correspondingtoaspecificactivityof2.5×105units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Note:Slowtodissolve.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
