Type
Recombinant
Description
IL-12 isadisulfide-linkedheterodimericprotein(p70),composedoftwosubunits,p35andp40,whichareencodedbytwodifferentgenes.Accumulatingdataindicatesthatp40secretionprecedesthatofIL-12 expression.Inaddition,toitsABIlitytocovalentlybindtop35toformIL-12,p40canbindtop19toformIL-23,oritcanformthehomodimerdesignatedIL-12 p80.ElevatedlevelsofIL-12 p80correlatetomacrophagerecruitmentandincreasedinflammationinasthmaandrespiratoryviralinfectionmodels.RecombinantHumanIL-12 p80isan80.0 kDadisulfidelinkedhomodimerconsistingoftwop40chainsof IL-12.
Source
(BTI-Tn-5B1-4)Hi-5Insectcells*
Purity
95%
BIOLOGicalActivity
Datanotavailable.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C